Deleted
Sample Discontinued
Experience: None
Not Used
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Composite

Part:BBa_K3292003

Designed by: José Arnulfo Juárez Figueroa   Group: iGEM19_Tec-Monterrey   (2019-10-16)


6His-tagged Super Folding GFP with terminator

This part is an improvement of the BBa_I746916 which allows you to choose which is the better option for your protein either to purify it with an affinity column or just to take it from the medium.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 874
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 875
    Illegal PstI site found at 889
    Illegal NotI site found at 882
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 875
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 875
    Illegal PstI site found at 889
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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